KMID : 0604520130390040259
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Journal of the Society of Cosmetic Scientists of Korea 2013 Volume.39 No. 4 p.259 ~ p.269
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Screening of Effective Extraction Conditions for Increasing Antioxidant Activities of Licorice Extracts from Various Countries of Origin
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Ha Ji-Hoon
Lee Hye-Mi Kwon Soon-Sik Kim Hae-Soo Kim Moon-Jin Jeon So-Ha Jeong Yoo-Min Hwang Jun-Pil Park Jong-Ho Choi Yung-Key Park Jin-Oh Park Soo-Nam Park Dong-Sik
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Abstract
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In this work, licorice extracts were prepared using various extraction conditions such as extraction solvent,temperature, and time from Glycyrrhiza uralensis (G. uralensis) produced in Korea and China and Glycyrrhiza glabra(G. glabra) in Uzbekistan. The optimum extraction condition was selected from the extraction yields and antioxidativeactivities of extracts. Korea licorice extracts showed the highest free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavengingactivity (46.05%) under the extraction condition of 85% ethanol at 60 ¡É for 6 hours. The prominent ROS(reactive oxygen species) scavenging activity using luminol-dependent chemiluminescence assay and the cellular protectiveeffect against1O2 induced cellular membrane damage were also shown from the extracts obtained from the same condition. Especially, Korea G. uralensis extracts exhibited the higher prominent protective effect (¥ó50 = 116.4 min)than (+)-¥á-tocopherol (¥ó50 = 28.5 min) and the extraction yield of Korea licorice extract was 18.75%, which is 1.2times and 2.5 times higher than that of Uzbekistan and China, respectively. These results indicate that the extractioncondition of 85% ethanol at 60 ¡É for 6 hours is optimal to prepare licorice extracts, which can be applicable as antioxidativecosmetic materials.
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KEYWORD
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Glycyrrhiza uralensis, Glycyrrhiza glabra, DPPH assay, luminol chemiluminescence assay, cellular protective effect
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